Novel technologies in doubled haploid line development

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Novel technologies in doubled haploid line development

haploid inducer line can be transferred (DH) technology can not only shorten the breeding process but also increase genetic gain. Haploid induction and subsequent genome doubling are the two main steps required for DH technology. Haploids have been generated through the culture of immature male and female gametophytes, and through inter- and intraspecific via chromosome elimination. Here, we fo...

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Quantitative genetics of doubled haploid populations and application to the theory of line development.

The line value of a genotype is defined as the expected value of all lines that can be derived from this genotype. Specific genetic effects are defined for this value: only additive and additive by additive epistatic effects are necessary. There is no dominance effect for such a value. A general expression for the covariances between related lines is given. From a design with several lines per ...

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Development of yellow rust resistant doubled haploid lines of wheat through wheat ´ maize Crosses

In order to produce doubled haploid lines of bread wheat resistant to strip or yellow rust, three F1 wheat hybrids were crossed using pollens of three maize hybrids. Out of 1071 pollinated florets, success in seed set ranged from 63.1% to 93.3% (mean 78%). Differences in seed set among the crosses were not significant. Embryo formation in the seeds developed on different crosses also varied fro...

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Mapping of Novel QTL Regulating Grain Shattering Using Doubled Haploid Population in Rice (Oryza sativa L.)

The critical evolutionary step during domestication of major cereals was elimination of seed shattering because the easy-to-shatter trait in wild relatives results in a severe reduction in yield. In this study, we analyzed the QTLs associated with shattering employing a high-density genetic map in doubled haploid (DH) population of rice (Oryza sativa L.). A genetic linkage map was generated wit...

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Haploid and Doubled Haploid Techniques in Perennial Ryegrass (Lolium perenne L.) to Advance Research and Breeding

The importance of haploid and doubled haploid (DH) techniques for basic and applied research, as well as to improve the speed of genetic gain when applied in breeding programs, cannot be overstated. They have become routine tools in several major crop species, such as maize (Zea mays L.), wheat (Triticum aestivum L.), and barley (Hordeum vulgare L.). DH techniques in perennial ryegrass (Lolium ...

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ژورنال

عنوان ژورنال: Plant Biotechnology Journal

سال: 2017

ISSN: 1467-7644,1467-7652

DOI: 10.1111/pbi.12805